blyscan dye Search Results


blyscan dye  (BioVendor Instruments)
91
BioVendor Instruments blyscan dye
FIGURE 2 Radiation stimulates vesicular CD44v3 expression. (A, <t>B)</t> <t>tEVs</t> from breast cancer cells were assessed for CD44v3 (A) and HS (B) expression via flow cytometry. Control represents unbound beads incubated with antibody cocktail. (C) tEVs from MDA-MB-231 cells were treated overnight with either heparanase, chondroitinase ABC, or both. Gag levels were assessed via <t>blyscan</t> assay. Data points represent technical replicates. (D) MRC5 were incubated with tEVs collected from WT or CD44 KO MDA-MB-231. IL-6 levels were measured via ELISA. (E) MDA-MB-231 were exposed to fresh media containing FCM described in (D) and radioresistance was assessed via clonogenic assay. X axis indicates which fibroblast CM from (D) was used in the incubation. Statistical significance for (A-E) assessed with ANOVA * = P < 0.05. (D, E) Data points represent biological replicates. ** denotes a P value < 0.005. *** denotes a P value < 0.0005. **** denotes a P value < 0.00005. These values were the results of ANOVA analyses performed on GraphPad. “ns” represents a value for P that is greater than 0.05 and stands for “Not Significant”.
Blyscan Dye, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blyscan+dye/Blyscan+Dye+Reagent/pm36106109-96-7-18
Average 91 stars, based on 1 article reviews
blyscan dye - by Bioz Stars, 2026-08
91/100 stars
  Buy from Supplier
blyscan dye reagent  (Accurate Chemical & Scientific Corporation)
90
Accurate Chemical & Scientific Corporation blyscan dye reagent
FIGURE 2 Radiation stimulates vesicular CD44v3 expression. (A, <t>B)</t> <t>tEVs</t> from breast cancer cells were assessed for CD44v3 (A) and HS (B) expression via flow cytometry. Control represents unbound beads incubated with antibody cocktail. (C) tEVs from MDA-MB-231 cells were treated overnight with either heparanase, chondroitinase ABC, or both. Gag levels were assessed via <t>blyscan</t> assay. Data points represent technical replicates. (D) MRC5 were incubated with tEVs collected from WT or CD44 KO MDA-MB-231. IL-6 levels were measured via ELISA. (E) MDA-MB-231 were exposed to fresh media containing FCM described in (D) and radioresistance was assessed via clonogenic assay. X axis indicates which fibroblast CM from (D) was used in the incubation. Statistical significance for (A-E) assessed with ANOVA * = P < 0.05. (D, E) Data points represent biological replicates. ** denotes a P value < 0.005. *** denotes a P value < 0.0005. **** denotes a P value < 0.00005. These values were the results of ANOVA analyses performed on GraphPad. “ns” represents a value for P that is greater than 0.05 and stands for “Not Significant”.
Blyscan Dye Reagent, supplied by Accurate Chemical & Scientific Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blyscan+dye/blyscan+dye+reagent/pm16326988-34-14-17
Average 90 stars, based on 1 article reviews
blyscan dye reagent - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
blyscan dye dissociation reagents  (BioVendor Instruments)
N/A
   Buy from Supplier

Image Search Results


FIGURE 2 Radiation stimulates vesicular CD44v3 expression. (A, B) tEVs from breast cancer cells were assessed for CD44v3 (A) and HS (B) expression via flow cytometry. Control represents unbound beads incubated with antibody cocktail. (C) tEVs from MDA-MB-231 cells were treated overnight with either heparanase, chondroitinase ABC, or both. Gag levels were assessed via blyscan assay. Data points represent technical replicates. (D) MRC5 were incubated with tEVs collected from WT or CD44 KO MDA-MB-231. IL-6 levels were measured via ELISA. (E) MDA-MB-231 were exposed to fresh media containing FCM described in (D) and radioresistance was assessed via clonogenic assay. X axis indicates which fibroblast CM from (D) was used in the incubation. Statistical significance for (A-E) assessed with ANOVA * = P < 0.05. (D, E) Data points represent biological replicates. ** denotes a P value < 0.005. *** denotes a P value < 0.0005. **** denotes a P value < 0.00005. These values were the results of ANOVA analyses performed on GraphPad. “ns” represents a value for P that is greater than 0.05 and stands for “Not Significant”.

Journal: Frontiers in oncology

Article Title: Radiation induces ESCRT pathway dependent CD44v3 + extracellular vesicle production stimulating pro-tumor fibroblast activity in breast cancer.

doi: 10.3389/fonc.2022.913656

Figure Lengend Snippet: FIGURE 2 Radiation stimulates vesicular CD44v3 expression. (A, B) tEVs from breast cancer cells were assessed for CD44v3 (A) and HS (B) expression via flow cytometry. Control represents unbound beads incubated with antibody cocktail. (C) tEVs from MDA-MB-231 cells were treated overnight with either heparanase, chondroitinase ABC, or both. Gag levels were assessed via blyscan assay. Data points represent technical replicates. (D) MRC5 were incubated with tEVs collected from WT or CD44 KO MDA-MB-231. IL-6 levels were measured via ELISA. (E) MDA-MB-231 were exposed to fresh media containing FCM described in (D) and radioresistance was assessed via clonogenic assay. X axis indicates which fibroblast CM from (D) was used in the incubation. Statistical significance for (A-E) assessed with ANOVA * = P < 0.05. (D, E) Data points represent biological replicates. ** denotes a P value < 0.005. *** denotes a P value < 0.0005. **** denotes a P value < 0.00005. These values were the results of ANOVA analyses performed on GraphPad. “ns” represents a value for P that is greater than 0.05 and stands for “Not Significant”.

Article Snippet: tEVs (50 mg) were stained with 1mL blyscan dye reagent for 30 minutes according to the manufacturers protocol (BioVendor R&D, Asheville, NC).

Techniques: Expressing, Cytometry, Control, Incubation, Enzyme-linked Immunosorbent Assay, Clonogenic Assay